Proteinase K CAS 39450-01-6

Proteinase K  Specification:

Proteinase K  Application Usage:

Proteinase K is widely used in molecular biological applications to remove protein contamination from preparations of highly native, undamaged nucleic acids. It rapidly and effectively inactivates nucleases that could degrade DNA or RNA, even in the presence of denaturing agents. Proteinase K remains active in 1% Triton X-100 and fully active in 0.5% (w/v) SDS, which helps denature protein substrates and increase digestion rates.
The enzyme works optimally at concentrations of 50-200 µg/mL, within a pH range of 7.5-11.0, and at temperatures between 37-70 °C. It is usually denatured by subsequent phenol extractions. Incubation times can vary from 30 minutes to 18 hours.
Proteinase K also exhibits autolysis, a process where the enzyme digests itself, particularly during prolonged incubation.

Proteinase K  Package and Shipping:

As customer request, non-danger goods.

Proteinase K  Storage:

recommends long term storage at -20 °C and keep dry for lyophilized powder to maintain stable for at least 3 years. Enzyme solutions prepared as recommended maintain stable at room temperature for 12 months.